Monthly Archives: June 2010

iGEM 2010 Update

Objective: Create an engineered bacteriophage that eliminates Staphylococcus aureus

To achieve  our objective we have divided the project into 6 tracks that will be pursued in parallel:

1. Biofilm- observe Staphylococcus aureus biofilm growth under lab conditions so we can characterize effectiveness of our engineered phage

2. DspB- extract the Dispersin B gene from A. pleuropneumoniae  so we can integrate into the phage genome

3. Phage- develop standard protocol to manipulate the phage genome in a simple and well-defined manner

4. Quorum- characterize the existing P2 biobrick part

5. Modelling- model the interaction between s. aureus biofilm, phage, and relevant molecules in the system, to supplement our wet lab experiment

6. Human Practices- gather feedback from public opinion of synthetic biology